Chromatography is a technique that is used to separate
mixtures. Each different mixture produces its own pattern called a chromatogram.
This process is often used
in crime labs to separate the components of substances found at the
scene of a crime.
For additional background of how and why this technique read the Chromatography
Links first to understand what you will be doing!
Materials:
- paper chromotography strips
- markers from suspects
- 250 ml beaker
- wood stick
- Chromotography fluid #1
- Chromotography fluid #2
- Chromotography fluid #3
Method:
A: preparing the Chromotography strip
- Trim the bottom of the Chromotography strip so that it is square
- Fold the strip so that it hangs just below the 50 ml mark on the
beaker
- Measure 1 cm from the bottom and draw a PENCIL line across the strip
- Use a suspect marker and put a small dot on to the chromotography
strip at the pencil line
B: Testing the marker dot
- Place 50 ml of chromatography fluid #1
- Fold the strip over the wood stick and
hang it in the beaker so the strip just touches the liquid
- Make sure the dot does ont touch or go under the liquid.
- Time for 12 minutes after the strip touches the liquid
- Observe
how the dot
separates into different colors on the strip.
- Dry the strip and tape it in your journal on page 18
- If the dot does not begin to seperate after a few minutes then prepare
another strip and follow part C
C: Testing the marker if Chromotography fluid #1 does not work
- Place 50 ml of chromatography fluid #2
- Fold the strip over the wood stick and hang it in the beaker so the
strip just touches the liquid
- Make sure the dot does ont touch or go under the liquid.
- Time for 12 minutes after the strip touches the liquid
- Observe how the dot separates into different colors on the strip.
- Dry the strip and tape it in your journal on page 18
- If the dot does not begin to seperate after a few minutes then prepare
another strip and follow this procedure again using Chromotography
fluid #3
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